Spectrophotometric determination of acyclovir after its reaction with ninhydrin and ascorbic acid
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KINETIC DETERMINATION OF ASCORBIC ACID a WITH SPECTROPHOTOMETRIC DETECTION
A simple and rapid method has been developed for the determination of ascorbic acid in fruit and pharmaceutical products. The method is based on the measurement of the reaction rate between ammonium molybdate and ascorbic acid in acidic media. The reaction was monitored spectrophotometrically at 800 nm by fixed-time and variable-time methods. The calibration graph was linear in the range of...
متن کاملSpectrophotometric determination of ascorbic acid and dehydroascorbic acid.
We present a method for measuring ascorbic acid in methanol/trichloroacetic acid extracts prepared from human plasma after enzymatic oxidation of ascorbic acid to dehydroascorbic acid by ascorbate oxidase. Samples were assayed by spectrophotometrically monitoring the kinetics of the concentration-dependent absorbance changes of dehydroascorbic acid with phosphate-citrate-methanol buffers. Ascor...
متن کاملkinetic determination of ascorbic acid a with spectrophotometric detection
a simple and rapid method has been developed for the determination of ascorbic acid in fruit and pharmaceutical products. the method is based on the measurement of the reaction rate between ammonium molybdate and ascorbic acid in acidic media. the reaction was monitored spectrophotometrically at 800 nm by fixed-time and variable-time methods. the calibration graph was linear in the range of 5 0...
متن کاملThe ninhydrin reaction and its analytical applications.
Feuge, R. O., Cousins, E. R., Fore, S. P., Dupre, E. F. & O'Connor, R. T. (1953). J. Amer. Oil Chem. Soc. 30, 454. Feuge, R. O., Pepper, M. B. jun., O'Connor, R. T. & Field, E. T. (1951). J. Amer. Oil Chem. Soc. 28, 420. Garton, G. A. (1954). J. Sci. Fd Agric. 5, 251. Grass Driers' Association (1941). Analyst, 66, 334. Hartman, L., Shorland, F. B. & McDonald, I. R. C. (1954). Nature, Lond., 174...
متن کاملThe quantitative determination of proline and pipecolic acid with ninhydrin.
In recent years, a number of photometric methods for the quantitative determination of ar-amino acids have been introduced (1, 2). These methods, based on reaction with ninhydrin in aqueous solution, are less satisfactory for the determination of imino acids.1 Grassmann and von Atnim (3) described reaction products of ninhydrin with imino acids and have suggested that these might be of use for ...
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ژورنال
عنوان ژورنال: Journal of Applied Pharmaceutical Science
سال: 2015
ISSN: 2231-3354
DOI: 10.7324/japs.2015.50411